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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to PCDH9

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别名:Protocadherin-9, PCDH9
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Description
This gene belongs to the protocadherin gene family, a
subfamily of the cadherin superfamily. The mRNA encodes a
cadherin-related neuronal receptor that localizes to synaptic
junctions and is putatively involved in specific neuronal
connections and signal transduction. Sharing a characteristic with
other protocadherin genes, this gene has a notably large exon that
encodes six cadherin domains and a transmembrane region. Two
alternatively spliced transcript variants encoding distinct
isoforms have been found for this gene.
Specification
Aliases Protocadherin-9, PCDH9
Entrez GeneID 5101
Swissprot Q9HC56
WB Predicted band size 136.1kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This PCDH9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1018-1046 amino acids from the C-terminal region of human PCDH9.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/2000
IHC 1/500

All lanes : Anti-PCDH9 Antibody (C-term) at 1:2000 dilution
Lane 1: 293 whole cell lysate
Lane 2: U-87 MG whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 136 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue using P34723 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34723 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。