Rabbit Polyclonal Antibody to PCDHA8

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

400-880-2824

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:Protocadherin alpha-8, PCDH-alpha-8, PCDHA8
应用:WB,IHC,ICC
反应种属:Human
规格：50μl/100μl

Description
This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.

Description

This gene is a member of the protocadherin alpha gene
cluster, one of three related gene clusters tandemly linked on
chromosome five that demonstrate an unusual genomic organization
similar to that of B-cell and T-cell receptor gene clusters. The
alpha gene cluster is composed of 15 cadherin superfamily genes
related to the mouse CNR genes and consists of 13 highly similar
and 2 more distantly related coding sequences. The tandem array of
15 N-terminal exons, or variable exons, are followed by downstream
C-terminal exons, or constant exons, which are shared by all genes
in the cluster. The large, uninterrupted N-terminal exons each
encode six cadherin ectodomains while the C-terminal exons encode
the cytoplasmic domain. These neural cadherin-like cell adhesion
proteins are integral plasma membrane proteins that most likely
play a critical role in the establishment and function of specific
cell-cell connections in the brain. Alternative splicing has been
observed and additional variants have been suggested but their
full-length nature has yet to be determined.

Specification
Aliases	Protocadherin alpha-8, PCDH-alpha-8, PCDHA8
Entrez GeneID	56140
Swissprot	Q9Y5H6
WB Predicted band size	103.1kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This PCDHA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 773-800 amino acids from the C-terminal region of human PCDHA8.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
ICC	1/10-1/50

	PCDHA8 Antibody (C-term) (Cat. #P31029) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PCDHA8 antibody detected the PCDHA8 protein (arrow).
	Confocal immunofluorescent analysis of PCDHA8 Antibody (C-term)(Cat#P31029) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	PCDHA8 Antibody (C-term) (Cat. #P31029)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHA8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.