Rabbit Polyclonal Antibody to PCDHAC2

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别名:Protocadherin alpha-C2, PCDH-alpha-C2, PCDHAC2
应用:WB,IHC,ICC,FCM
反应种属:Human
规格：50μl/100μl

Description
This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.

Description

This gene is a member of the protocadherin alpha gene
cluster, one of three related gene clusters tandemly linked on
chromosome five that demonstrate an unusual genomic organization
similar to that of B-cell and T-cell receptor gene clusters. The
alpha gene cluster is composed of 15 cadherin superfamily genes
related to the mouse CNR genes and consists of 13 highly similar
and 2 more distantly related coding sequences. The tandem array of
15 N-terminal exons, or variable exons, are followed by downstream
C-terminal exons, or constant exons, which are shared by all genes
in the cluster. The large, uninterrupted N-terminal exons each
encode six cadherin ectodomains while the C-terminal exons encode
the cytoplasmic domain. These neural cadherin-like cell adhesion
proteins are integral plasma membrane proteins that most likely
play a critical role in the establishment and function of specific
cell-cell connections in the brain. Alternative splicing has been
observed and additional variants have been suggested but their
full-length nature has yet to be determined.

Specification
Aliases	Protocadherin alpha-C2, PCDH-alpha-C2, PCDHAC2
Entrez GeneID	56134
Swissprot	Q9Y5I4
WB Predicted band size	109.4kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This PCDHAC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 616-644 amino acids from the Central region of human PCDHAC2.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
ICC	1/10-1/50
FCM	1/10-1/50

	PCDHAC2 Antibody (Center) (Cat. #P31045) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PCDHAC2 antibody detected the PCDHAC2 protein (arrow).
	Confocal immunofluorescent analysis of PCDHAC2 Antibody (Center)(Cat#P31045) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
	PCDHAC2 Antibody (Center) (Cat. #AP31967) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
	PCDHAC2 Antibody (Center) (Cat. #P31045)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHAC2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.