Rabbit Polyclonal Antibody to PCSK2 (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:Neuroendocrine convertase 2, NEC 2, KEX2-like endoprotease 2, Prohormone convertase 2, Proprotein convertase 2, PC2, PCSK2, NEC2
应用:WB,IHC,ICC,FCM
反应种属:Human, Mouse
规格：50μl/100μl

Description
PCSK2 belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This protein is a proinsulin-processing enzyme that plays a key role in regulating insulin biosynthesis. The protein is also known to cleave proopiomelanocortin, proenkephalin, prodynorphin and proluteinizing-hormone-releasing hormone.

Description

PCSK2 belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This protein is a proinsulin-processing enzyme that plays a key role in regulating insulin biosynthesis. The protein is also known to cleave proopiomelanocortin, proenkephalin, prodynorphin and proluteinizing-hormone-releasing hormone.

Specification
Aliases	Neuroendocrine convertase 2, NEC 2, KEX2-like endoprotease 2, Prohormone convertase 2, Proprotein convertase 2, PC2, PCSK2, NEC2
Entrez GeneID	5126
Swissprot	P16519
WB Predicted band size	70.6kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	This PCSK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-116 amino acids from the N-terminal region of human PCSK2.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Application
WB	1/1000
IHC	1/100-1/500
ICC	1/10-1/50
FCM	1/10-1/50

	Anti-PCSK2 Antibody (N-term) at 1:1000 dilution + TT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 71 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	Western blot analysis of PCSK2 Antibody (N-term) (Cat. #P33664) in mouse cerebellum tissue lysates (35ug/lane). PCSK2 (arrow) was detected using the purified Pab.
	Confocal immunofluorescent analysis of PCSK2 Antibody (N-term)(Cat#P33664) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	PCSK2 Antibody (N-term) (Cat. #P33664) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human brain tissue reacted with PCSK2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.