中文 
别名:ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE-2应用:WB,IHC,ICC
反应种属:Human,Mouse,Rat
规格：50μl/100μl
| Specification | |
|---|---|
| Aliases | ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE-2 |
| Entrez GeneID | 2077; |
| Swissprot | P50548 |
| WB Predicted band size | 90kDa |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PE2. |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Application | |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| ICC | 1/100-1/300 |
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Western blot analysis of PE2 (phospho-Thr526) expression in HEK293, MCF7, mouse lung, rat lung whole cell lysates. |
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Immunofluorescent analysis of PE2 (phospho-Thr526) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
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Immunohistochemical analysis of PE2 (phospho-Thr526) staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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