Rabbit Polyclonal Antibody to PEMT

包装: 1G|100G|500G|1KG|100KG

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别名:PLMT; PNMT; PEAMT; PEMPT; PEMT2
应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl

Description
Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

Description

Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

Specification
Aliases	PLMT; PNMT; PEAMT; PEMPT; PEMT2
Swissprot	Q9UBM1
WB Predicted band size	22 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Synthetic peptide of human PEMT
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/1000-1/5000
IHC	1/30-1/150
ELISA	1/5000-1/10000

	Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-4: Mouse liver tissue, HepG2, 293T and 231 cell lysates, Primary antibody: P06778(PEMT Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second
	The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P06778(PEMT Antibody) at dilution 1/35. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P06778(PEMT Antibody) at dilution 1/35. (Original magnification: ×200)