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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to PHC3

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别名:Polyhomeotic-like protein 3, Early development regulatory protein 3, Homolog of polyhomeotic 3, hPH3, PHC3, EDR3, PH3
应用:WB
反应种属:Human, Mouse
规格:50μl/100μl

Description
Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A ‘Lys-119’, rendering chromatin heritably changed in its expressibility.
Specification
Aliases Polyhomeotic-like protein 3, Early development regulatory protein 3, Homolog of polyhomeotic 3, hPH3, PHC3, EDR3, PH3
Entrez GeneID 80012
Swissprot Q8NDX5
WB Predicted band size 106.2kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This PHC3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 819-850 amino acids from human PHC3.
Application
WB 1/2000

All lanes : Anti-PHC3 Antibody (C-Term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: SH-SY5Y whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: U-2OS whole cell lysate
Lane 5: A549 whole cell lysate
Lane 6: MCF-7 whole cell lysate
Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 106 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

P33495 staining PHC3 in human kidney tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。