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Rabbit Polyclonal Antibody to PHF5A

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别名:PHD finger-like domain-containing protein 5A, PHD finger-like domain protein 5A, Splicing factor 3B-associated 14 kDa protein, SF3b14b, PHF5A
应用:WB,FCM
反应种属:Human
规格:50μl/100μl

Description
This gene encodes a subunit of the splicing factor 3b
protein complex. Splicing factor 3b, together with splicing factor
3a and a 12S RNA unit, forms the U2 small nuclear
ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a
complex binds pre-mRNA upstream of the intron’s branch site in a
sequence-independent manner and may anchor the U2 snRNP to the
pre-mRNA. The protein encoded by this gene contains a
PHD-finger-like domain that is flanked by highly basic N- and
C-termini. This protein belongs to the PHD-finger superfamily and
may act as a chromatin-associated protein. This gene has several
pseudogenes on different chromosomes.
Specification
Aliases PHD finger-like domain-containing protein 5A, PHD finger-like domain protein 5A, Splicing factor 3B-associated 14 kDa protein, SF3b14b, PHF5A
Entrez GeneID 84844
Swissprot Q7RTV0
WB Predicted band size 12.4kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This PHF5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the C-terminal region of human PHF5A.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
FCM 1/10-1/50

All lanes : Anti-PHF5A Antibody (C-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HL-60 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 12 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

PHF5A Antibody (C-term) (Cat. #P33737) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。