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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to PHO1 (N-term)

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别名:DNA dC->dU-editing enzyme APOBEC-3A, A3A, 354-, Phorbolin-1, APOBEC3A
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Description
PHO1 a member of the cytidine deaminase gene family. The PHO1 gene is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. This gene encodes a protein that lacks the zinc binding activity and may be an expressed pseudogene.
Specification
Aliases DNA dC->dU-editing enzyme APOBEC-3A, A3A, 354-, Phorbolin-1, APOBEC3A
Entrez GeneID 100913187;200315
Swissprot P31941
WB Predicted band size 23.0kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This PHO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PHO1.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/2000
IHC 1/100-1/500

All lanes : Anti-hPHO1-M1 at 1:2000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: Raji whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 23 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of hPHO1-M1 (Cat. #P34661) in Jurkat cell line lysates (35ug/lane). PHO1 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PHO1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。