别名:DNA dC->dU-editing enzyme APOBEC-3A, A3A, 354-, Phorbolin-1, APOBEC3A应用:WB,IHC
反应种属:Human
规格:50μl/100μl
Description |
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PHO1 a member of the cytidine deaminase gene family. The PHO1 gene is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. This gene encodes a protein that lacks the zinc binding activity and may be an expressed pseudogene. |
Specification | |
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Aliases | DNA dC->dU-editing enzyme APOBEC-3A, A3A, 354-, Phorbolin-1, APOBEC3A |
Entrez GeneID | 100913187;200315 |
Swissprot | P31941 |
WB Predicted band size | 23.0kDa |
Host/Isotype | Rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This PHO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PHO1. |
Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Application | |
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WB | 1/2000 |
IHC | 1/100-1/500 |
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All lanes : Anti-hPHO1-M1 at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Western blot analysis of hPHO1-M1 (Cat. #P34661) in Jurkat cell line lysates (35ug/lane). PHO1 (arrow) was detected using the purified Pab. |
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Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PHO1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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