Rabbit Polyclonal Antibody to Phospho-4E BP1 (Thr37)

包装: 1G|100G|500G|1KG|100KG

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别名:EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
应用:WB,IHC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity.

Specification
Aliases	EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Entrez GeneID	1978
Swissprot	Q13541
WB Predicted band size	Calculated MW: 13 kDa; Observed MW: 18 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Synthetic peptide of human EIF4EBP1
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
WB	1/500-1/1000
IHC	1/50-1/100

	Western blot analysis of Phospho-4E BP1 (Thr37) in MDAMB435 lysates using Phospho-4E BP1 (Thr37) antibody.
	Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma, using 4EBP1 (Phospho-Thr36) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right.