Rabbit Polyclonal Antibody to Phospho-AMPK alpha 1/2 (Thr183/Thr172)

包装: 1G|100G|500G|1KG|100KG

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别名:PRKAA1; AMPK1; 5′ -AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK
应用:WB,IHC,ICC
反应种属:Human,Mouse,Rat,Monkey,Pig
规格：50μl/100μl

Description
AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2; β1, 2; γ1, 2, 3).

Specification
Aliases	PRKAA1; AMPK1; 5′ -AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK
Entrez GeneID	5562/5563
Swissprot	Q13131/P54646
WB Predicted band size	Calculated MW: 64 kDa; Observed MW: 64 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat,Monkey,Pig
Immunogen	The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200
ELISA	1/10000

	Western blot analysis of Phospho-AMPK alpha 1/2 (Thr183/Thr172) in various lysates using Phospho-AMPK alpha 1/2 (Thr183/Thr172) antibody.
	Western blot analysis of Phospho-AMPK alpha 1/2 (Thr183/Thr172) in KB lysates using Phospho-AMPKα1/2 (T183/17, 2) antibody.
	Western blot analysis of Phospho-AMPK alpha 1/2 in rat muscle heart using Phospho-AMPK alpha 1/2 antibody.
	Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-AMPK alpha 1/2 (Thr183/Thr172) antibody.High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody. was preabsorbed by immunogen peptide.
	Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-AMPK alpha 1/2 (Thr183/Thr172) antibody.High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval.Negetive contrl (right) obtaned from antibody was preabsorbed by immunogen peptide.