Rabbit Polyclonal Antibody to Phospho-Artemis (Ser516)

包装: 1G|100G|500G|1KG|100KG

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别名:SCIDA; SNM1C; A-SCID; RS-SCID; DCLREC1C
应用:WB,IHC
反应种属:Human,Mouse
规格：50μl/100μl

Description
Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5′-3′ exonuclease activity in isolation and acquires endonucleolytic activity on 5′ and 3′ hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.

Description

Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5′-3′ exonuclease activity in isolation and acquires endonucleolytic activity on 5′ and 3′ hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.

Specification
Aliases	SCIDA; SNM1C; A-SCID; RS-SCID; DCLREC1C
Entrez GeneID	64421
Swissprot	Q96SD1
WB Predicted band size	Calculated MW: 78 kDa; Observed MW: 78 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	The antiserum was produced against synthesized peptide derived from human Artemis around the phosphorylation site of Ser516. AA range:482-531
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
WB	1/500-1/1000
IHC	1/50-1/100
ELISA	1/10000

	Western blot analysis of Phospho-Artemis (Ser516) in HepG2 cells treated with EGF , using Phospho-Artemis (Ser516) antibody.The lane on the right is blocked with the Phospho- peptide.
	Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma, using Phospho-Artemis (Ser516) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.