Rabbit Polyclonal Antibody to Phospho-Cardiac Troponin I (Ser22/Ser23)

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别名:TNNI3; TNNC1; Troponin I; cardiac muscle; Cardiac troponin I
应用:WB,IHC
反应种属:Mouse,Rat
规格：50μl/100μl

Description
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

Description

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

Specification
Aliases	TNNI3; TNNC1; Troponin I; cardiac muscle; Cardiac troponin I
Entrez GeneID	29248
Swissprot	P23693
WB Predicted band size	Calculated MW: 24 kDa; Observed MW: 28 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Mouse,Rat
Immunogen	The antiserum was produced against synthesized peptide derived from rat TNNI3 around the phosphorylation site of Ser22 and Ser23. AA range:5-54
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
WB	1/500-1/1000
IHC	1/50-1/100
ELISA	1/10000

	EnzymeLinked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phospho-peptide (Phospho-left) and NonPhospho-peptide (Phospho-right), using TNNI3 (Phospho-Ser22+Ser23) antibody.
	Western blot analysis of Phospho-Cardiac Troponin I (Ser22/Ser23) in SH-SY5Y lysates using Phospho-Cardiac Troponin I (Ser22/Ser23) antibody.
	Western blot analysis of Phospho-Cardiac Troponin I (Ser22/Ser23) in mouse heart lysates using Phospho-Cardiac Troponin I (Ser22/Ser23) antibody.The lane on the right is blocked with the Phospho- peptide.
	Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle using TNNI3 (Phospho-Ser22+Ser23) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.