Rabbit Polyclonal Antibody to Phospho-EGFR(Y1016)

包装: 1G|100G|500G|1KG|100KG

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别名:Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
应用:WB
反应种属:Human
规格：50μl/100μl

Description
Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. EGFR is a receptor for EGF, but also for other members of the EGF family, including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. It is involved in the control of cell growth and differentiation.

Description

Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. EGFR is a receptor for EGF, but also for other members of the EGF family, including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. It is involved in the control of cell growth and differentiation.

Specification
Aliases	Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Entrez GeneID	1956
Swissprot	P00533
WB Predicted band size	134.3kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This EGFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1016 of human EGFR.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Application
WB	1/500-1/8000

	Dot blot analysis of anti-EGFR B1 Phospho-specific Pab (Cat.#P30250) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
	Western blot analysis of lysate from A431 cells(from left to right),untreated or treated with EGF at 100ng/ml,using Phospho-EGFR-pY1016 Antibody (Cat. #P30250) or EGFR-pS695 Antibody.Lysate at 15ug per lane.P30250 was diluted at 1:8000 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.