Rabbit Polyclonal Antibody to Phospho-ErbB 2 (Tyr1221/Tyr1222)

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:ERBB2; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD antigen CD340
应用:WB,IHC,IP
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.

Description

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.

Specification
Aliases	ERBB2; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD antigen CD340
Entrez GeneID	2064
Swissprot	P04626
WB Predicted band size	Calculated MW: 138 kDa; Observed MW: 180 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr1221/Tyr1222. AA range:1191-1240
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ELISA	1/10000

	Western blot analysis of Phospho-ErbB 2 (Tyr1221/Tyr1222) in various lysates using Phospho-ErbB 2 (Tyr1221/Tyr1222) antibody.
	Western blot analysis of Phospho-ErbB 2 (Tyr1221/Tyr1222) in SKOV-3 lysates treated with EGF, using HER2 (Phospho-Tyr1221/Tyr122, 2) antibody. The lane on the right is blocked with the Phospho- peptide.
	Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma, using HER2 (Phospho-Tyr1221/Tyr122, 2) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right.