Rabbit Polyclonal Antibody to Phospho-IKK beta (Tyr188)

包装: 1G|100G|500G|1KG|100KG

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别名:IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB
应用:WB,IHC
反应种属:Human,Mouse,Rat,Monkey
规格：50μl/100μl

Description
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits.

Description

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits.

Specification
Aliases	IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB
Entrez GeneID	3551
Swissprot	O14920
WB Predicted band size	Calculated MW: 87 kDa; Observed MW: 87 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat,Monkey
Immunogen	The antiserum was produced against synthesized peptide derived from human IKK-beta around the phosphorylation site of Tyr188. AA range:161-210
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
WB	1/500-1/1000
IHC	1/50-1/100
ELISA	1/10000

	EnzymeLinked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phospho-peptide (Phospho-left) and NonPhospho-peptide (Phospho-right), using IKKbeta (Phospho-Tyr18antibody
	Western blot analysis of Phospho-IKK beta (Tyr188) in COS7 lysates using Phospho-IKK beta (Tyr188) antibody.The lane on the right is blocked with the synthesized peptide.
	Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-IKK beta (Tyr188) antibody.High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval.Sample with blocking peptide on the right.
	Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Phospho-IKK beta (Tyr188) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.