Rabbit Polyclonal Antibody to Phospho-IRAK1(S376)

包装: 1G|100G|500G|1KG|100KG

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别名:Interleukin-1 receptor-associated kinase 1, IRAK-1, IRAK1, IRAK
应用:WB,ICC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
IRAK1 binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. This protein is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B.

Description

IRAK1 binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. This protein is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B.

Specification
Aliases	Interleukin-1 receptor-associated kinase 1, IRAK-1, IRAK1, IRAK
Entrez GeneID	3654
Swissprot	P51617
WB Predicted band size	76.5kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This IRAK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S376 of human IRAK1.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	DB: 1/500
ICC	1/10-1/50

	Dot blot analysis of anti-hIRAK1-pS376 Phospho-specific Pab (Cat. #P30126) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
	Confocal immunofluorescent analysis of Phospho-IRAK1-S376 Antibody(Cat#P30126) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	Dot blot analysis of anti-hIRAK1-pS376 Phospho-specific Pab (Cat. #P30126) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.