Rabbit Polyclonal Antibody to Phospho-MYC(T58)

包装: 1G|100G|500G|1KG|100KG

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别名:Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64, MYC, BHLHE39
应用:WB
反应种属:Human
规格：50μl/100μl

Description
MYC participates in the regulation of gene transcription. It binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5′-CAC[GA]TG-3′. This protein appears to activate the transcription of growth-related genes. Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia.

Description

MYC participates in the regulation of gene transcription. It binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5′-CAC[GA]TG-3′. This protein appears to activate the transcription of growth-related genes. Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia.

Specification
Aliases	Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64, MYC, BHLHE39
Entrez GeneID	4609
Swissprot	P01106
WB Predicted band size	48.8kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This MYC Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T58 of human MYC.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/500-1/1000

	Dot blot analysis of Phospho-MYC-T58 Pab (Cat.P30208) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
	Western blot analysis of Phospho-MYC-T58 Antibody in human TPA activated Hela cell line lysates. Phospho-MYC (arrow) was detected using the purified PAb. (0: without TPA; 1: 60ug/ml TPA, 15min; 2: 60ug/ml TPA, 30min; 3: 60ug/ml TPA, 45min; 4: 125ug/ml TPA, 15min; 5: 125ug/ml TPA, 30min; 6: 125ug/ml TPA, 45min; 7: 250ug/ml TPA, 15min; 8: 250ug/ml TPA, 30min; 9: 250ug/ml, 45min)