Rabbit Polyclonal Antibody to Phospho-Rb (Ser780)

包装: 1G|100G|500G|1KG|100KG

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别名:RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
应用:WB,IHC,ICC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

Description

Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

Specification
Aliases	RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
Entrez GeneID	5925
Swissprot	P06400
WB Predicted band size	Calculated MW: 106 kDa; Observed MW: 106 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser780. AA range:751-800
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200
ELISA	1/10000

	Western blot analysis of Phospho-Rb (Ser780) in various lysates using Phospho-Rb (S780) antibody.
	Western blot analysis of Phospho-Rb (Ser780) in K562 lysates treated with serum 10% using Phospho-Rb (Ser780) antibody.The lane on the right is blocked with the Phospho- peptide.
	Western blot analysis of Phospho-Rb (Ser780) in KB lysates using Phospho-Rb (S780) antibody.
	Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Retinoblastoma (Phospho-Ser78) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.