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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to Phospho-RB(S608)

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别名:Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Description
Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression (‘cure’) occurs in some cases.
Specification
Aliases Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1
Entrez GeneID 5925
Swissprot P06400
WB Predicted band size 106.2kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This RB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S608 of human RB.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Application
WB DB: 1/500
IHC 1/100-1/500

Dot blot analysis of anti-Phospho-RB-S608 Antibody (Cat.#P30153) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。