中文 
别名:Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1应用:WB,IHC,ICC
反应种属:Human
规格：50μl/100μl
| Description |
|---|
| Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression occurs in some cases. |
| Specification | |
|---|---|
| Aliases | Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1 |
| Entrez GeneID | 5925 |
| Swissprot | P06400 |
| WB Predicted band size | 106.2kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Rb Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S811 of human Rb. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Application | |
|---|---|
| WB | DB: 1/500 |
| IHC | 1/100-1/500 |
| ICC | 1/10-1/50 |
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Dot blot analysis of anti-hRb-S811 Phospho-specific Pab (Cat. #P30154) on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml. |
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Confocal immunofluorescent analysis of Phospho-Rb-S811 Antibody(Cat#P30154) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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