Rabbit Polyclonal Antibody to Phospho-SEPARIN(S1126)

包装: 1G|100G|500G|1KG|100KG

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别名:Separin, Caspase-like protein ESPL1, Extra spindle poles-like 1 protein, Separase, ESPL1, ESP1, KIAA0165
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
The metaphase-to-anaphase transition is the final discrete event in duplication and separation of the genetic material of the cell. Its timing is regulated by the activation of the anaphase-promoting complex (APC). In both budding and fission yeast, the degradation of the Pds1 or Cut2 protein, respectively, is required for the onset of sister chromatid separation. Both proteins are APC substrates. Pds1 and Cut2 proteins associate with the yeast separin proteins Esp1 and Cut1, respectively, and prevent the separins from promoting chromatid separation. Pds1 and Cut2 are also called anaphase inhibitors or securins

Description

The metaphase-to-anaphase transition is the final discrete event in duplication and separation of the genetic material of the cell. Its timing is regulated by the activation of the anaphase-promoting complex (APC). In both budding and fission yeast, the degradation of the Pds1 or Cut2 protein, respectively, is required for the onset of sister chromatid separation. Both proteins are APC substrates. Pds1 and Cut2 proteins associate with the yeast separin proteins Esp1 and Cut1, respectively, and prevent the separins from promoting chromatid separation. Pds1 and Cut2 are also called anaphase inhibitors or securins

Specification
Aliases	Separin, Caspase-like protein ESPL1, Extra spindle poles-like 1 protein, Separase, ESPL1, ESP1, KIAA0165
Entrez GeneID	9700
Swissprot	Q14674
WB Predicted band size	233.2kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This SEPARIN Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1126 of human SEPARIN.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	DB: 1/500
IHC	1/100-1/500

	Dot blot analysis of anti-hSeparase-S801 Phospho-specific Pab (Cat. #P30155) on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.