Rabbit Polyclonal Antibody to Phospho-SMAD4(T277)

包装: 1G|100G|500G|1KG|100KG

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别名:Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, Smad4, hSMAD4, SMAD4, DPC4, MADH4
应用:WB,IHC,ICC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily, SMAD4 is the common SMAD (co-SMAD). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.

Specification
Aliases	Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, Smad4, hSMAD4, SMAD4, DPC4, MADH4
Entrez GeneID	4089
Swissprot	Q13485
WB Predicted band size	60.4kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This SMAD4 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T277 of human SMAD4.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	DB: 1/500
IHC	1/100-1/500
ICC	1/10-1/50

	Dot blot analysis of anti-Phospho-SMAD4-T277 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
	Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with λ phosphatase, using Phospho-SMAD4 Antibody (T277)(upper) or GAPDH (lower).
	Confocal immunofluorescent analysis of Phospho-SMAD4-T277 Antibody(Cat#P30236) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	Formalin-fixed and paraffin-embedded Breast Carcinoma reacted with SMAD4-T277 (center) (Cat.#P30236), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.