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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to PI15 (N-term)

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别名:Peptidase inhibitor 15, PI-15, 25 kDa trypsin inhibitor, p25TI, Cysteine-rich secretory protein 8, CRISP-8, SugarCrisp, PI15, CRISP8, P25TI
应用:WB,IHC,FCM
反应种属:Human
规格:50μl/100μl

Description
This gene encodes a trypsin inhibitor. The protein shares
similarity to insect venom allergens, mammalian testis-specific
proteins and plant pathogenesis-related proteins. It is frequently
expressed in human neuroblastoma and glioblastoma cell lines, and
thus may play a role in the central nervous system. [provided by
RefSeq].
Specification
Aliases Peptidase inhibitor 15, PI-15, 25 kDa trypsin inhibitor, p25TI, Cysteine-rich secretory protein 8, CRISP-8, SugarCrisp, PI15, CRISP8, P25TI
Entrez GeneID 51050
Swissprot O43692
WB Predicted band size 29.1kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This PI15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-63 amino acids from the N-terminal region of human PI15.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
IHC 1/100-1/500
FCM 1/10-1/50

All lanes : Anti-PI15 Antibody (N-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: PC-3 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 29 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

PI15 Antibody (N-term) (Cat. #P33904) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PI15 antibody detected the PI15 protein (arrow).
PI15 Antibody (N-term) (Cat. #P33904) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
PI15 Antibody (N-term) (Cat. #P33904)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PI15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。