Rabbit Polyclonal Antibody to PI3-kinase p85- alpha (Phospho-Tyr607)

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别名:p85, AGM7, GRB1, p85-ALPHA, PIK3R1
应用:WB,IHC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.

Description

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.

Specification
Aliases	p85, AGM7, GRB1, p85-ALPHA, PIK3R1
Entrez GeneID	5295;
Swissprot	P27986
WB Predicted band size	80kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Peptide sequence around phosphorylation site of Tyrosine 607 (D-Q-Y(p)-S-L) derived from Human PI3-kinase p85-alpha.
Formulation	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/200-1/500
IHC	1/50-1/100

	All Lanes: PI3-kinase p85- (Phospho-Tyr607) Antibody at 1/ 500 dilution. Lane1: MCF7 serum starvation Lane2: MCF7 treated with 20nM EGF for 15min Lane3:MCF7 treated with 100nM Dasatinib for 30min Lane4: MCF7 Serum Starvation, then treated with Lambda Protein Phosphatase Lane5: MCF7 treated with 20nM EGF for 15min, then treated with Lambda Protein Phosphatase Lane6:MCF7 treated with 100nM Dasatinib for 30min, then treated with Lambda Protein Phosphatase Lysates/ Proteins at 40ug per lane Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution Predicted band size : 80kDa Observed band size:80kDa
	Lane1: Rat kidney tissue lysate Lane2: Rat kidney tissue lysate, blocked with the phospho peptide
	Immunohistochemical analysis of paraffin-embedded Human placenta. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
	Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemistry analysis of paraffin-embedded human brain, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The picture on the right is blocked with the phospho peptide.