Rabbit Polyclonal Antibody to PIAS2

包装: 1G|100G|500G|1KG|100KG

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别名:ARIP3; Androgen receptor-interacting protein 3; DAB2-interacting protein; DIP; Miz1
应用:WB
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at’Lys-65′ and ‘Lys-160’.Christina-Maria Hecker, J. Biol. Chem., Jun 2006; 281: 16117 – 16127. Daria Donati, J. Immunol., SepHeitham Hassoun, 2006; 177: 3035 – 3044. Heitham Hassoun, Am J Physiol Renal Physiol, Feb 2007; 10.1152.David J. Sharkey, Mol. Hum. Reprod., Jul 2007;

Description

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at’Lys-65′ and ‘Lys-160’.Christina-Maria Hecker, J. Biol. Chem., Jun 2006; 281: 16117 – 16127. Daria Donati, J. Immunol., SepHeitham Hassoun, 2006; 177: 3035 – 3044. Heitham Hassoun, Am J Physiol Renal Physiol, Feb 2007; 10.1152.David J. Sharkey, Mol. Hum. Reprod., Jul 2007;

Specification
Aliases	ARIP3; Androgen receptor-interacting protein 3; DAB2-interacting protein; DIP; Miz1
Entrez GeneID	9063;
Swissprot	O75928
WB Predicted band size	68kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from Internal of human PIAS2.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000

	Western blot analysis of extracts from COS7 cells, using PIAS2 antibody P41841.
	Western blot analysis of extracts from HuvEc cells (Lane 2), JK cells (Lane 3) and cos-7 cells (Lane 4), using PIAS2 antiobdy P41841. The lane on the left is treated with synthesized peptide.