中文 
别名:Piwi-like RNA-mediated gene silencing 4, HIWI2; MIWI2应用:WB
反应种属:Human
规格：50μl/100μl
| Description |
|---|
| PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells. Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. |
| Specification | |
|---|---|
| Aliases | Piwi-like RNA-mediated gene silencing 4, HIWI2; MIWI2 |
| Entrez GeneID | 143689; |
| Swissprot | Q7Z3Z4 |
| WB Predicted band size | 97kDa |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein corresponding to a region derived from 546-838 amino acids of human piwi-like RNA-mediated gene silencing 4 |
| Formulation | Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol. |
| Application | |
|---|---|
| WB | 1/500-1/2000 |
| ELISA | 1:2000-1:5000 |
 |
Gel: 8%SDS-PAGELane1: Hela cell lysateLane2: 231 cell lysate Lane3: 293T cell lysateLysates: 40 ug per lanePrimary antibody: 1/550 dilutionSecondary antibody: Goat anti Rabbit IgG – H&L (HRP) at 1/10000 dilutionExposure time: 10 seconds |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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