Rabbit Polyclonal Antibody to PKC zeta

包装: 1G|100G|500G|1KG|100KG

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别名:PRKCZ; PKC2; Protein kinase C zeta type; nPKC-zeta
应用:WB,IHC,ICC
反应种属:Human,Mouse,Rat,Monkey
规格：50μl/100μl

Description
PKC is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.

Description

PKC is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.

Specification
Aliases	PRKCZ; PKC2; Protein kinase C zeta type; nPKC-zeta
Entrez GeneID	5590
Swissprot	Q05513
WB Predicted band size	Calculated MW: 68 kDa; Observed MW: 80 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat,Monkey
Immunogen	The antiserum was produced against synthesized peptide derived from human PKC zeta. AA range:526-575
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200
ELISA	1/10000

	Western blot analysis of PKC zeta in various lysates using PKC zeta antibody.
	Western blot analysis of PKC zeta in COS7 lysates using PKC ζ antibody.
	Western blot analysis of PKC zeta in COS7 lytsates treated with PMA using PKC zeta antibody. The lane on the right is blocked with the synthesized peptide.
	Immunofluorescence analysis of PKC zeta in.NIH/3T3 cells, using PKC zeta antibody. The picture on the right is blocked with the synthesized peptide.
	Immunohistochemistry analysis of paraffin-embedded Human brain tissue using PKC zeta antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.