Rabbit Polyclonal Antibody to PMS2

包装: 1G|100G|500G|1KG|100KG

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别名:DNA mismatch repair gene; DNA mismatch repair protein PMS2; HNPCC4; PMS1 protein homolog 2
应用:WB,IHC,ICC,FCM,IP
反应种属:Human
规格：50μl/100μl

Description
Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.

Description

Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.

Specification
Aliases	DNA mismatch repair gene; DNA mismatch repair protein PMS2; HNPCC4; PMS1 protein homolog 2
Entrez GeneID	5395
Swissprot	P54278
WB Predicted band size	Calculated MW: 96 kDa; Observed MW: 110 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	A synthesized peptide derived from human PMS2
Formulation	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200
FCM	1/50-1/100

	Western blot analysis of PMS2 in (1) Jurkat lysates; (2)HeLa lysates using PMS2 antibody.
	Immunohistochemistry analysis of paraffin-embedded Human tonsil using PMS2 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.