Rabbit Polyclonal Antibody to POLG

包装: 1G|100G|500G|1KG|100KG

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别名:DNA polymerase subunit gamma-1, Mitochondrial DNA polymerase catalytic subunit, PolG-alpha, POLG, MDP1, POLG1, POLGA
应用:WB,IHC,ICC,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene.

Description

Mitochondrial DNA polymerase is heterotrimeric, consisting
of a homodimer of accessory subunits plus a catalytic subunit. The
protein encoded by this gene is the catalytic subunit of
mitochondrial DNA polymerase. The encoded protein contains a
polyglutamine tract near its N-terminus that may be polymorphic.
Defects in this gene are a cause of progressive external
ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory
ataxic neuropathy dysarthria and ophthalmoparesis (SANDO),
Alpers-Huttenlocher syndrome (AHS), and mitochondrial
neurogastrointestinal encephalopathy syndrome (MNGIE). Two
transcript variants encoding the same protein have been found for
this gene.

Specification
Aliases	DNA polymerase subunit gamma-1, Mitochondrial DNA polymerase catalytic subunit, PolG-alpha, POLG, MDP1, POLG1, POLGA
Entrez GeneID	5428
Swissprot	P54098
WB Predicted band size	139.6kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This POLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1120-1148 amino acids from the C-terminal region of human POLG.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
ICC	1/10-1/50
FCM	1/10-1/50

	POLG Antibody (C-term) (Cat. #P31564) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the POLG antibody detected the POLG protein (arrow).
	Confocal immunofluorescent analysis of POLG Antibody (C-term)(Cat#P31564) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	POLG Antibody (C-term) (Cat. #P31564) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	POLG Antibody (C-term) (Cat. #P31564)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.