别名:DNA polymerase subunit gamma-2, mitochondrial, DNA polymerase gamma accessory 55 kDa subunit, p55, Mitochondrial DNA polymerase accessory subunit, MtPolB, PolG-beta, POLG2, MTPOLB应用:WB,IHC
反应种属:Human
规格:50μl/100μl
Description |
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The accuracy of mitochondrial DNA (mtDNA) replication depends on the coordinated action of many nuclear-encoded proteins and on the correct balance of nucleotides within the mitochondrial matrix. mtDNA is replicated by DNA polymerase gamma, which is composed of a 140-kD catalytic subunit(POLG1) and a 55-kD accessory subunit (POLG2). |
Specification | |
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Aliases | DNA polymerase subunit gamma-2, mitochondrial, DNA polymerase gamma accessory 55 kDa subunit, p55, Mitochondrial DNA polymerase accessory subunit, MtPolB, PolG-beta, POLG2, MTPOLB |
Entrez GeneID | 11232 |
Swissprot | Q9UHN1 |
WB Predicted band size | 54.9kDa |
Host/Isotype | Rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This POLG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205-234 amino acids from the Central region of human POLG2. |
Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Application | |
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WB | 1/1000 |
IHC | 1/100-1/500 |
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Anti-POLG2 Antibody (Center) at 1:1000 dilution + HL-60 whole cell lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Western blot analysis of anti-POLG2 Antibody (Center) (Cat.#P34190) in K562 cell line lysates (35ug/lane). POLG2 (arrow) was detected using the purified Pab. |
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Formalin-fixed and paraffin-embedded human brain tissue reacted with POLG2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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