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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to PRL

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别名:Prolactin, PRL, PRL
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Description
PRL prolactin acts primarily on the mammary gland by promoting lactation.
Specification
Aliases Prolactin, PRL, PRL
Entrez GeneID 5617
Swissprot P01236
WB Predicted band size 25.9kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This PRL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-76 amino acids from the Central region of human PRL.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
IHC 1/100

All lanes : Anti-PRL Antibody (Center) at 1:1000 dilution
Lane 1: Daudi whole cell lysate
Lane 2: U-87 MG whole cell lysate
Lane 3: SH-SY5Y whole cell lysate
Lane 4: MCF-7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 26 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of P34774 on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of P34774 on paraffin-embedded Human placenta tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。