Rabbit Polyclonal Antibody to PSMA5

包装: 1G|100G|500G|1KG|100KG

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别名:Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome zeta chain, PSMA5
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PMA5 is a member of the peptidase T1A family, that is a 20S core alpha subunit.

Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PMA5 is a member of the peptidase T1A family, that is a 20S core alpha subunit.

Specification
Aliases	Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome zeta chain, PSMA5
Entrez GeneID	5686
Swissprot	P28066
WB Predicted band size	26.4kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This PSMA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the Central region of human PSMA5.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Application
WB	1/1000
IHC	1/100-1/500

	Western blot analysis of PSMA5 (arrow) using rabbit polyclonal PSMA5 Antibody (Center) (Cat.#P30187). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2) (Origene Technologies).
	Western blot analysis of PSMA5 Antibody (Center) (Cat.#P30187) in HL60 cell line lysates (35ug/lane). PSMA5(arrow) was detected using the purified polyclonal antibody.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PSMA5 antibody (Center) (Cat.#P30187), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.