Rabbit Polyclonal Antibody to PSMB5

包装: 1G|100G|500G|1KG|100KG

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别名:Proteasome subunit beta type-5, Macropain epsilon chain, Multicatalytic endopeptidase complex epsilon chain, Proteasome chain 6, Proteasome epsilon chain, Proteasome subunit MB1, Proteasome subunit X, PSMB5, LMPX, MB1, X
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene.

Description

The proteasome is a multicatalytic proteinase complex with
a highly ordered ring-shaped 20S core structure. The core structure
is composed of 4 rings of 28 non-identical subunits; 2 rings are
composed of 7 alpha subunits and 2 rings are composed of 7 beta
subunits. Proteasomes are distributed throughout eukaryotic cells
at a high concentration and cleave peptides in an
ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
essential function of a modified proteasome, the immunoproteasome,
is the processing of class I MHC peptides. This gene encodes a
member of the proteasome B-type family, also known as the T1B
family, that is a 20S core beta subunit in the proteasome. This
catalytic subunit is not present in the immunoproteasome and is
replaced by catalytic subunit 3i (proteasome beta 8 subunit).
Multiple transcript variants encoding different isoforms have been
found for this gene.

Specification
Aliases	Proteasome subunit beta type-5, Macropain epsilon chain, Multicatalytic endopeptidase complex epsilon chain, Proteasome chain 6, Proteasome epsilon chain, Proteasome subunit MB1, Proteasome subunit X, PSMB5, LMPX, MB1, X
Entrez GeneID	5693
Swissprot	P28074
WB Predicted band size	28.5kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This PSMB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-263 amino acids from the C-terminal region of human PSMB5.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500

	PSMB5 Antibody (C-term) (Cat. #P31488) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PSMB5 antibody detected the PSMB5 protein (arrow).
	Western blot analysis of PSMB5 (arrow) using rabbit polyclonal PSMB5 Antibody (C-term) (Cat. #P31488). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PSMB5 gene.
	PSMB5 Antibody (C-term) (P31488)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PSMB5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.