中文 
别名:26S proteasome non-ATPase regulatory subunit 3, 26S proteasome regulatory subunit RPN3, 26S proteasome regulatory subunit S3, Proteasome subunit p58, PSMD3应用:WB,IHC,FCM
反应种属:Human, Mouse
规格：50μl/100μl
| Description |
|---|
| Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. |
| Specification | |
|---|---|
| Aliases | 26S proteasome non-ATPase regulatory subunit 3, 26S proteasome regulatory subunit RPN3, 26S proteasome regulatory subunit S3, Proteasome subunit p58, PSMD3 |
| Entrez GeneID | 5709 |
| Swissprot | O43242 |
| WB Predicted band size | 61.0kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This PSMD3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 482-515 amino acids from human PSMD3. |
| Application | |
|---|---|
| WB | 1/2000 |
| IHC | 1/250 |
| FCM | 1/25 |
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All lanes : Anti-PSMD3 Antibody (C-Term) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing HeLa cells stained with P34443(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34443, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
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P34443 staining PSMD3 in human brain tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
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P34443 staining PSMD3 in human testis tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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