Rabbit Polyclonal Antibody to PTPRC (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
应用:WB,FCM
反应种属:Human
规格：50μl/100μl

Description
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq].

Description

The protein encoded by this gene is a member of the
protein tyrosine phosphatase (PTP) family. PTPs are known to be
signaling molecules that regulate a variety of cellular processes
including cell growth, differentiation, mitotic cycle, and
oncogenic transformation. This PTP contains an extracellular
domain, a single transmembrane segment and two tandem
intracytoplasmic catalytic domains, and thus belongs to receptor
type PTP. This gene is specifically expressed in hematopoietic
cells. This PTP has been shown to be an essential regulator of T-
and B-cell antigen receptor signaling. It functions through either
direct interaction with components of the antigen receptor
complexes, or by activating various Src family kinases required for
the antigen receptor signaling. This PTP also suppresses JAK
kinases, and thus functions as a regulator of cytokine receptor
signaling. Four alternatively spliced transcripts variants of this
gene, which encode distinct isoforms, have been reported. [provided
by RefSeq].

Specification
Aliases	Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
Entrez GeneID	5788
Swissprot	P08575
WB Predicted band size	147.5kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This PTPRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids of human PTPRC.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
FCM	1/10-1/50

	All lanes : Anti-PTPRC Antibody (N-term) at 1:1000 dilution Lane 1: CCRF-CEM whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 180kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	Anti-PTPRC Antibody (N-term) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 147 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	PTPRC Antibody (N-term) (Cat. #P35005) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PTPRC antibody detected the PTPRC protein (arrow).
	PTPRC Antibody (N-term) (Cat. #P35005) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.