Rabbit Polyclonal Antibody to PUM2 (N-term)

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别名:Pumilio homolog 2, Pumilio-2, PUM2, KIAA0235, PUMH2
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
PUM2 is a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. This protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3′ untranslated regions. The evolutionarily conserved function of this protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation.

Description

PUM2 is a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. This protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3′ untranslated regions. The evolutionarily conserved function of this protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation.

Specification
Aliases	Pumilio homolog 2, Pumilio-2, PUM2, KIAA0235, PUMH2
Entrez GeneID	23369
Swissprot	Q8TB72
WB Predicted band size	114.2kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This PUM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-162 amino acids from the N-terminal region of human PUM2.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500

	Western blot analysis of PUM2 (arrow) using rabbit polyclonal PUM2 Antibody (N-term) (Cat.#P30314). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human testis tissue reacted with PUM2 Antibody (C-term) (Cat.#P30314), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.