Rabbit Polyclonal Antibody to PUR4 (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:Phosphoribosylformylglycinamidine synthase, FGAM synthase, FGAMS, Formylglycinamide ribonucleotide amidotransferase, FGAR amidotransferase, FGAR-AT, Formylglycinamide ribotide amidotransferase, PFAS, KIAA0361
应用:WB,IHC,FCM
反应种属:Human
规格：50μl/100μl

Specification
Aliases	Phosphoribosylformylglycinamidine synthase, FGAM synthase, FGAMS, Formylglycinamide ribonucleotide amidotransferase, FGAR amidotransferase, FGAR-AT, Formylglycinamide ribotide amidotransferase, PFAS, KIAA0361
Entrez GeneID	5198
Swissprot	O15067
WB Predicted band size	144.7kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This PUR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-213 amino acids from the N-terminal region of human PUR4.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	All lanes : Anti-PUR4 Antibody (N-term) at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 145 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	Western blot analysis of PUR4 Antibody (N-term) (Cat. #P33442) in HepG2 cell line lysates (35ug/lane). PUR4 (arrow) was detected using the purified Pab.
	PUR4 Antibody (N-term) (Cat. #P33442) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	PUR4 Antibody (N-term) (Cat. #P33442) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PUR4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.