Rabbit Polyclonal Antibody to RAD17

包装: 1G|100G|500G|1KG|100KG

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别名:Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog, RAD17, R24L
应用:WB,FCM
反应种属:Human
规格：50μl/100μl

Description
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

Description

The protein encoded by this gene is highly similar to the
gene product of Schizosaccharomyces pombe rad17, a cell cycle
checkpoint gene required for cell cycle arrest and DNA damage
repair in response to DNA damage. This protein shares strong
similarity with DNA replication factor C (RFC), and can form a
complex with RFCs. This protein binds to chromatin prior to DNA
damage and is phosphorylated by the checkpoint kinase ATR following
damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein
complex onto chromatin after DNA damage, which may be required for
its phosphorylation. The phosphorylation of this protein is
required for the DNA-damage-induced cell cycle G2 arrest, and is
thought to be a critical early event during checkpoint signaling in
DNA-damaged cells. Eight alternatively spliced transcript variants
of this gene, which encode four distinct proteins, have been
reported. Two pseudogenes, located on chromosomes 7 and 13, have
been identified.

Specification
Aliases	Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog, RAD17, R24L
Entrez GeneID	5884
Swissprot	O75943
WB Predicted band size	77.1kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This RAD17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human RAD17.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
FCM	1/10-1/50

	RAD17 Antibody (Center) (Cat. #P30990) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RAD17 antibody detected the RAD17 protein (arrow).
	RAD17 Antibody (Center) (Cat. #P30990) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.