Rabbit Polyclonal Antibody to RAD50

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别名:NBSLD; RAD502; hRad50
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

Description

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

Specification
Aliases	NBSLD; RAD502; hRad50
Swissprot	Q92878
WB Predicted band size	154 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide of human RAD50
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/500-1/2000
IHC	1/50-1/200
ELISA	1/2000-1/10000

	Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-5: 293T cells, Hela cells, K562 cells, NIH/3T3 cells, RAW264.7 cells, Primary antibody: P04189(RAD50 Antibody) at dilution 1/900, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
	The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using P04189(RAD50 Antibody) at dilution 1/40. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P04189(RAD50 Antibody) at dilution 1/40. (Original magnification: ×200)