Rabbit Polyclonal Antibody to Rad9 (S387)

包装: 1G|100G|500G|1KG|100KG

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别名:Cell cycle checkpoint control protein RAD9A, hRAD9, DNA repair exonuclease rad9 homolog A, RAD9A
应用:WB,IHC,ICC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3′ to 5′ exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene.

Description

This gene product is highly similar to Schizosaccharomyces
pombe rad9, a cell cycle checkpoint protein required for cell cycle
arrest and DNA damage repair in response to DNA damage. This
protein is found to possess 3′ to 5′ exonuclease activity, which
may contribute to its role in sensing and repairing DNA damage. It
forms a checkpoint protein complex with RAD1 and HUS1. This complex
is recruited by checkpoint protein RAD17 to the sites of DNA
damage, which is thought to be important for triggering the
checkpoint-signaling cascade. Use of alternative polyA sites has
been noted for this gene.

Specification
Aliases	Cell cycle checkpoint control protein RAD9A, hRAD9, DNA repair exonuclease rad9 homolog A, RAD9A
Entrez GeneID	5883
Swissprot	Q99638
WB Predicted band size	42.5kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This Rad9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-391 amino acids from human Rad9.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
ICC	1/10-1/50

	Rad9 Antibody (pS387) (Cat. #P30968) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Rad9 antibody detected the Rad9 protein (arrow).
	Confocal immunofluorescent analysis of Rad9 Antibody (S387)(Cat#P30968) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	Rad9 Antibody (S387) (Cat. #P30968)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Rad9 Antibody (S387) for immunohistochemistry. Clinical relevance has not been evaluated.