Rabbit Polyclonal Antibody to RARA

包装: 1G|100G|500G|1KG|100KG

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别名:Retinoic acid receptor alpha, RAR-alpha, Nuclear receptor subfamily 1 group B member 1, RARA, NR1B1
应用:WB,IHC,ICC
反应种属:Human, Mouse
规格：50μl/100μl

Description
This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.

Description

This gene represents a nuclear retinoic acid receptor. The
encoded protein, retinoic acid receptor alpha, regulates
transcription in a ligand-dependent manner. This gene has been
implicated in regulation of development, differentiation,
apoptosis, granulopoeisis, and transcription of clock genes.
Translocations between this locus and several other loci have been
associated with acute promyelocytic leukemia. Alternatively spliced
transcript variants have been found for this locus.

Specification
Aliases	Retinoic acid receptor alpha, RAR-alpha, Nuclear receptor subfamily 1 group B member 1, RARA, NR1B1
Entrez GeneID	5914
Swissprot	P10276
WB Predicted band size	50.8kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	This RARA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-349 amino acids from the C-terminal region of human RARA.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
ICC	1/10-1/50

	All lanes : Anti-RARA Antibody (C-term) at 1:1000 dilution Lane 1: HL-60 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	All lanes : Anti-RARA Antibody (C-term) at 1:1000 dilution Lane 1: HL-60 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	RARA Antibody (C-term) (Cat. #P34655) western blot analysis in HL-60 cell line and mouse testis tissue lysates (35ug/lane).This demonstrates the RARA antibody detected the RARA protein (arrow).
	Confocal immunofluorescent analysis of RARA Antibody (C-term) (Cat#P34655) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	RARA Antibody (C-term) (Cat. #P34655)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RARA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.