Rabbit Polyclonal Antibody to Rb

包装: 1G|100G|500G|1KG|100KG

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别名:RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
应用:WB,IHC,ICC,IP
反应种属:Human,Mouse
规格：50μl/100μl

Description
Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

Description

Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

Specification
Aliases	RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
Entrez GeneID	5925
Swissprot	P06400
WB Predicted band size	Calculated MW: 106 kDa; Observed MW: 106 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	A synthesized peptide derived from human Retinoblastoma
Formulation	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of Retinoblastoma in (1) Jurkat lysates; (2) MCF-7 lysates using Rb antibody.
	Immunofluorescence analysis of Rb in SH-SY5Y using Retinoblastoma antibody.
	Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Retinoblastoma antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.