Rabbit Polyclonal Antibody to RFA2 (Ab-21)

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别名:REPA2; RF-A; RP-A; RPA2; RPA32
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage the RPA complex controls DNA repair and DNA damage checkpoint activation. It is required for the recruitment of the DNA double-strand break repair factors RAD52 and RAD51 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP which is involved in replication fork restart to sites of DNA damage. May also play a role in telomere maintenance.Erdile L.F., J. Biol. Chem. 265:3177-3182(1990).Gregory S.G., Nature 441:315-321(2006).Niu H., J. Biol. Chem. 272:12634-12641(1997).Cho J.M., Nucleic Acids Res. 28:3478-3485(2000)

Description

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage the RPA complex controls DNA repair and DNA damage checkpoint activation. It is required for the recruitment of the DNA double-strand break repair factors RAD52 and RAD51 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP which is involved in replication fork restart to sites of DNA damage. May also play a role in telomere maintenance.Erdile L.F., J. Biol. Chem. 265:3177-3182(1990).Gregory S.G., Nature 441:315-321(2006).Niu H., J. Biol. Chem. 272:12634-12641(1997).Cho J.M., Nucleic Acids Res. 28:3478-3485(2000)

Specification
Aliases	REPA2; RF-A; RP-A; RPA2; RPA32
Entrez GeneID	6118;
Swissprot	P15927
WB Predicted band size	32kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized non-phosphopeptide derived from human RFA2 around the phosphorylation site of threonine 21 (G-Y-T(p)-Q-S).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from K562 cells, using RFA2 (Ab-21) antibody P41666.
	Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using RFA2 (Ab-21) antiobdy P41666.