Rabbit Polyclonal Antibody to RhoA/B/C

包装: 1G|100G|500G|1KG|100KG

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别名:ARH12; ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC
应用:WB,IHC,ICC,FCM
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
RhoA,RhoB,RhoC is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. The three mammalian Rho proteins (A, B and C) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

Description

RhoA,RhoB,RhoC is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. The three mammalian Rho proteins (A, B and C) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

Specification
Aliases	ARH12; ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC
Entrez GeneID	387/388/389
Swissprot	P61586/P62745/P08134
WB Predicted band size	Calculated MW: 22 kDa; Observed MW: 22 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	A synthesized peptide derived from human Rho A + B + C
Formulation	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200
FCM	1/50-1/100

	Western blot analysis of Rho A + B + C expression in (1) Jurkat lysates; (2) NIH/3T3 lysates using RhoA/B/C antibody
	Western blot analysis of Rho A + B + C in SH-SY5Y and rat Brain lysates using Rho A + B + C antibody.
	Immunofluorescent analysis of RhoA/B/C in Hela using RhoA/B/C antibody.
	Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma using Rho A + B + C antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.