Rabbit Polyclonal Antibody to RIPK2 (Phospho-Ser176)

包装: 1G|100G|500G|1KG|100KG

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别名:CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta converting enzyme associated kinase; CARDIAK; EC 2.7.11.1; kinase RIPK2; Receptor-interacting protein 2; Receptor-interacting serine/threonine protein kinase 2; RICK; RIP-2; RIP-like
应用:WB,IHC
反应种属:Human,Mouse
规格：50μl/100μl

Description
Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes ‘Lys-63’-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces ‘Lys-63’-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

Description

Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes ‘Lys-63’-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces ‘Lys-63’-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

Specification
Aliases	CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta converting enzyme associated kinase; CARDIAK; EC 2.7.11.1; kinase RIPK2; Receptor-interacting protein 2; Receptor-interacting serine/threonine protein kinase 2; RICK; RIP-2; RIP-like
Entrez GeneID	8767;
Swissprot	O43353
WB Predicted band size	62kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	Peptide sequence around phosphorylation site of serine 176 (S-L-S(p)-Q-S) derived from Human RIPK2.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from 293 cells, treated with UV (15mins), using RIPK2 (Phospho-Ser176) antibody P40725. The lane on the right is treated with the synthesized peptide.
	Immunohistochemistry analysis of paraffin-embedded human brain tissue using RIPK2 (Phospho-Ser176) antibody P40725. The picture on the right is treated with the synthesized peptide.