Rabbit Polyclonal Antibody to RPA2

包装: 1G|100G|500G|1KG|100KG

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别名:REPA2; RPA32; RP-A p32; RP-A p34
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Replication protein A 32 kDa subunit is a protein that in humans is encoded by theRPA2 gene. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.

Description

Replication protein A 32 kDa subunit is a protein that in humans is encoded by theRPA2 gene. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.

Specification
Aliases	REPA2; RPA32; RP-A p32; RP-A p34
Swissprot	P15927
WB Predicted band size	29 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Full length fusion protein
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/500-1/2000
IHC	1/30-1/150
ELISA	1/2000-1/5000

	Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-7: Hela cells, HUVEC cells, HepG2 cells, A549 cells, Jurkat cells, 231 cells, A431 cells, Primary antibody: P01748(RPA2 Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
	The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P01748(RPA2 Antibody) at dilution 1/45. (Original magnification: ×200)