Rabbit Polyclonal Antibody to RPL10 (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:60S ribosomal protein L10, Laminin receptor homolog, Protein QM, Tumor suppressor QM, RPL10, DXS648E, QM
应用:WB
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L10E family of ribosomal proteins. It is located in the cytoplasm. In vitro studies have shown that the chicken protein can bind to c-Jun and can repress c-Jun-mediated transcriptional activation, but these activities have not been demonstrated in vivo. This gene was initially identified as a candidate for a Wilms tumor suppressor gene, but later studies determined that this gene is not involved in the suppression of Wilms tumor. This gene has been referred to as ‘laminin receptor homolog’ because a chimeric transcript consisting of sequence from this gene and sequence from the laminin receptor gene was isolated; however, it is not believed that this gene encodes a laminin receptor. Transcript variants utilizing alternative polyA signals exist. The variant with the longest 3′ UTR overlaps the deoxyribonuclease I-like 1 gene on the opposite strand. This gene is co-transcribed with the small nucleolar RNA gene U70, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Description

Ribosomes, the organelles that catalyze protein synthesis,
consist of a small 40S subunit and a large 60S subunit. Together
these subunits are composed of 4 RNA species and approximately 80
structurally distinct proteins. This gene encodes a ribosomal
protein that is a component of the 60S subunit. The protein belongs
to the L10E family of ribosomal proteins. It is located in the
cytoplasm. In vitro studies have shown that the chicken protein can
bind to c-Jun and can repress c-Jun-mediated transcriptional
activation, but these activities have not been demonstrated in
vivo. This gene was initially identified as a candidate for a Wilms
tumor suppressor gene, but later studies determined that this gene
is not involved in the suppression of Wilms tumor. This gene has
been referred to as ‘laminin receptor homolog’ because a chimeric
transcript consisting of sequence from this gene and sequence from
the laminin receptor gene was isolated; however, it is not believed
that this gene encodes a laminin receptor. Transcript variants
utilizing alternative polyA signals exist. The variant with the
longest 3′ UTR overlaps the deoxyribonuclease I-like 1 gene on the
opposite strand. This gene is co-transcribed with the small
nucleolar RNA gene U70, which is located in its fifth intron. As is
typical for genes encoding ribosomal proteins, there are multiple
processed pseudogenes of this gene dispersed through the genome.

Specification
Aliases	60S ribosomal protein L10, Laminin receptor homolog, Protein QM, Tumor suppressor QM, RPL10, DXS648E, QM
Entrez GeneID	6134
Swissprot	P27635
WB Predicted band size	24.6kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This RPL10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human RPL10.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/2000

All lanes : Anti-RPL10 Antibody (N-term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: Daudi whole cell lysate
Lane 3: U266B1 whole cell lysate
Lane 4: 293 whole cell lysate
Lane 5: NIH/3T3 whole cell lysate
Lane 6: Rat pancreas lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 25 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。

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