别名:60S ribosomal protein L15, RPL15, EC45应用:WB,IHC,FCM
反应种属:Human, Mouse
规格:50μl/100μl
Description |
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Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15E family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with the yeast ribosomal protein YL10 gene. Although this gene has been referred to as RPL10, its official symbol is RPL15. This gene has been shown to be overexpressed in some esophageal tumors compared to normal matched tissues. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
Specification | |
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Aliases | 60S ribosomal protein L15, RPL15, EC45 |
Entrez GeneID | 6138 |
Swissprot | P61313 |
WB Predicted band size | 24.1kDa |
Host/Isotype | Rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse |
Immunogen | This RPL15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human RPL15. |
Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Application | |
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WB | 1/1000 |
IHC | 1/100-1/500 |
FCM | 1/10-1/50 |
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Anti-RPL15 Antibody (N-term) at 1:1000 dilution + HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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RPL15 Antibody (N-term) (Cat. #P33611) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis. |
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RPL15 Antibody (N-term) (Cat. #P33611)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPL15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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