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Rabbit Polyclonal Antibody to RPL15 (N-term)

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别名:60S ribosomal protein L15, RPL15, EC45
应用:WB,IHC,FCM
反应种属:Human, Mouse
规格:50μl/100μl

Description
Ribosomes, the organelles that catalyze protein synthesis,
consist of a small 40S subunit and a large 60S subunit. Together
these subunits are composed of 4 RNA species and approximately 80
structurally distinct proteins. This gene encodes a ribosomal
protein that is a component of the 60S subunit. The protein belongs
to the L15E family of ribosomal proteins. It is located in the
cytoplasm. This gene shares sequence similarity with the yeast
ribosomal protein YL10 gene. Although this gene has been referred
to as RPL10, its official symbol is RPL15. This gene has been shown
to be overexpressed in some esophageal tumors compared to normal
matched tissues. Transcript variants utilizing alternative polyA
signals exist. As is typical for genes encoding ribosomal proteins,
there are multiple processed pseudogenes of this gene dispersed
through the genome.
Specification
Aliases 60S ribosomal protein L15, RPL15, EC45
Entrez GeneID 6138
Swissprot P61313
WB Predicted band size 24.1kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This RPL15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human RPL15.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
IHC 1/100-1/500
FCM 1/10-1/50

Anti-RPL15 Antibody (N-term) at 1:1000 dilution + HepG2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 24 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

RPL15 Antibody (N-term) (Cat. #P33611) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
RPL15 Antibody (N-term) (Cat. #P33611)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPL15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。