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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to RPL18A

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别名:60S ribosomal protein L18a, RPL18A
应用:WB,IHC,FCM
反应种属:Human, Mouse
规格:50μl/100μl

Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL18A is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18AE family of ribosomal proteins. It is located in the cytoplasm.
Specification
Aliases 60S ribosomal protein L18a, RPL18A
Entrez GeneID 6142
Swissprot Q02543
WB Predicted band size 20.8kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This RPL18A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-166 amino acids from the C-terminal region of human RPL18A.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
IHC 1/100-1/500
FCM 1/10-1/50

All lanes : Anti-RPL18A Antibody (C-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: MOLT-4 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 21 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of RPL18A Antibody (C-term) (Cat. #P33877) in Hela, 293 cell line lysates (35ug/lane). RPL18A (arrow) was detected using the purified Pab.
RPL18A Antibody (C-term) (Cat. #P33877) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human brain tissue reacted with RPL18A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。